OrangerV1, Main, Exploration, bibRecord, 001F57

Genetic transformation of Citrus sinensis cv. Hamlin with hrpN gene from Erwinia amylovora and evaluation of the transgenic lines for resistance to citrus canker

Identifieur interne : 001F57 ( Main/Exploration ); précédent : 001F56; suivant : 001F58

Genetic transformation of Citrus sinensis cv. Hamlin with hrpN gene from Erwinia amylovora and evaluation of the transgenic lines for resistance to citrus canker

Auteurs : Janaynna Magalhaes Barbosa-Mendes [Brésil] ; Francisco Filho De Assis Alves Mourao [Brésil] ; Armando Filho Bergamin [Brésil] ; Ricardo Harakava [Brésil] ; Steven V. Beer [États-Unis] ; Beatriz Madalena Januzzi Mendes [Brésil]

Source :

Scientia horticulturae [ 0304-4238 ] ; 2009.

RBID : Pascal:09-0317660

Descripteurs français

Pascal (Inist)
- Transformation génétique, Gène, Plante transgénique, Bactériose, Horticulture, Citrus sinensis, Erwinia amylovora, Agrume, Xanthomonas axonopodis, Résistance aux maladies, Chancre plante, Harpine.
Wicri :
- topic : Plante transgénique, Horticulture, Agrume.

English descriptors

KwdEn :
- Bacteriosis, Citrus fruit, Citrus sinensis, Disease resistance, Erwinia amylovora, Gene, Genetic transformation, Harpin, Horticulture, Plant cankers, Transgenic plant, Xanthomonas axonopodis.

Abstract

Transgenic Citrus sinensis (L.) Osb. cv. Hamlin plants expressing the hrpN gene were obtained by Agrobacterium tumefaciens (Smith and Towns) Conn-mediated transformation. hrpN encodes a harpin protein, which elicits the hypersensitive response and systemic acquired resistance in plants. The gene construct consisted of gst1, a pathogen-inducible promoter, a signal peptide for protein secretion to the apoplast, the selection genes nptII or aacC1 and the Nos terminator. The function of gst1 in citrus was evaluated in transgenic C. sinensis cv. Valencia harboring the reporter gene uidA (gus) driven by this promoter. Histochemical analysis for gus revealed that gst1 is activated in citrus leaves by both wounding and inoculation with Xanthomonas axonopodis Starr and Garces pv. citri (Hasse) Vauterin et al. Genetic transformation was confirmed by Southern blot hybridization in eight cv. Hamlin acclimatized plants. RT-PCR confirmed hrpN gene expression in seven cv. Hamlin transgenic lines before pathogen inoculation. Some hrpN transgenic lines showed severe leaf curling and abnormal growth. Six hrpN transgenic lines were propagated and evaluated for susceptibility to X. axonopodis pv. citri. RT-PCR confirmed gene expression in all six hrpN transgenic lines after pathogen inoculation. Several of the hrpN transgenic lines showed reduction in susceptibility to citrus canker as compared with non-transgenic plants. One hrpN transgenic line exhibited normal vegetative development and displayed very high resistance to the pathogen, estimated as up to 79% reduction in disease severity. This is the first report of genetic transformation of citrus using a pathogen-inducible promoter and the hrpN gene. Further evaluations of the transgenic plants under field conditions are planned. Nevertheless, the evidence to date suggests that the hrpN gene reduces the susceptibility of citrus plants to the canker disease.

Affiliations:

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Le document en format XML

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<author><name sortKey="Januzzi Mendes, Beatriz Madalena" sort="Januzzi Mendes, Beatriz Madalena" uniqKey="Januzzi Mendes B" first="Beatriz Madalena" last="Januzzi Mendes">Beatriz Madalena Januzzi Mendes</name>
<affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Centro de Energia Nuclear na Agricultura, Universidade de São Paulo</s1>
<s2>13400-970 Piracicaba, SP</s2>
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<series><title level="j" type="main">Scientia horticulturae</title>
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<term>Citrus fruit</term>
<term>Citrus sinensis</term>
<term>Disease resistance</term>
<term>Erwinia amylovora</term>
<term>Gene</term>
<term>Genetic transformation</term>
<term>Harpin</term>
<term>Horticulture</term>
<term>Plant cankers</term>
<term>Transgenic plant</term>
<term>Xanthomonas axonopodis</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Transformation génétique</term>
<term>Gène</term>
<term>Plante transgénique</term>
<term>Bactériose</term>
<term>Horticulture</term>
<term>Citrus sinensis</term>
<term>Erwinia amylovora</term>
<term>Agrume</term>
<term>Xanthomonas axonopodis</term>
<term>Résistance aux maladies</term>
<term>Chancre plante</term>
<term>Harpine</term>
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<front><div type="abstract" xml:lang="en">Transgenic Citrus sinensis (L.) Osb. cv. Hamlin plants expressing the hrpN gene were obtained by Agrobacterium tumefaciens (Smith and Towns) Conn-mediated transformation. hrpN encodes a harpin protein, which elicits the hypersensitive response and systemic acquired resistance in plants. The gene construct consisted of gst1, a pathogen-inducible promoter, a signal peptide for protein secretion to the apoplast, the selection genes nptII or aacC1 and the Nos terminator. The function of gst1 in citrus was evaluated in transgenic C. sinensis cv. Valencia harboring the reporter gene uidA (gus) driven by this promoter. Histochemical analysis for gus revealed that gst1 is activated in citrus leaves by both wounding and inoculation with Xanthomonas axonopodis Starr and Garces pv. citri (Hasse) Vauterin et al. Genetic transformation was confirmed by Southern blot hybridization in eight cv. Hamlin acclimatized plants. RT-PCR confirmed hrpN gene expression in seven cv. Hamlin transgenic lines before pathogen inoculation. Some hrpN transgenic lines showed severe leaf curling and abnormal growth. Six hrpN transgenic lines were propagated and evaluated for susceptibility to X. axonopodis pv. citri. RT-PCR confirmed gene expression in all six hrpN transgenic lines after pathogen inoculation. Several of the hrpN transgenic lines showed reduction in susceptibility to citrus canker as compared with non-transgenic plants. One hrpN transgenic line exhibited normal vegetative development and displayed very high resistance to the pathogen, estimated as up to 79% reduction in disease severity. This is the first report of genetic transformation of citrus using a pathogen-inducible promoter and the hrpN gene. Further evaluations of the transgenic plants under field conditions are planned. Nevertheless, the evidence to date suggests that the hrpN gene reduces the susceptibility of citrus plants to the canker disease.</div>
</front>
</TEI>
<affiliations><list><country><li>Brésil</li>
<li>États-Unis</li>
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<li>État de São Paulo</li>
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<settlement><li>Ithaca (New York)</li>
<li>São Paulo</li>
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<orgName><li>Université Cornell</li>
<li>Université de São Paulo</li>
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<tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Magalhaes Barbosa Mendes, Janaynna" sort="Magalhaes Barbosa Mendes, Janaynna" uniqKey="Magalhaes Barbosa Mendes J" first="Janaynna" last="Magalhaes Barbosa-Mendes">Janaynna Magalhaes Barbosa-Mendes</name>
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<name sortKey="Bergamin, Armando Filho" sort="Bergamin, Armando Filho" uniqKey="Bergamin A" first="Armando Filho" last="Bergamin">Armando Filho Bergamin</name>
<name sortKey="De Assis Alves Mourao, Francisco Filho" sort="De Assis Alves Mourao, Francisco Filho" uniqKey="De Assis Alves Mourao F" first="Francisco Filho" last="De Assis Alves Mourao">Francisco Filho De Assis Alves Mourao</name>
<name sortKey="Harakava, Ricardo" sort="Harakava, Ricardo" uniqKey="Harakava R" first="Ricardo" last="Harakava">Ricardo Harakava</name>
<name sortKey="Januzzi Mendes, Beatriz Madalena" sort="Januzzi Mendes, Beatriz Madalena" uniqKey="Januzzi Mendes B" first="Beatriz Madalena" last="Januzzi Mendes">Beatriz Madalena Januzzi Mendes</name>
</country>
<country name="États-Unis"><region name="État de New York"><name sortKey="Beer, Steven V" sort="Beer, Steven V" uniqKey="Beer S" first="Steven V." last="Beer">Steven V. Beer</name>
</region>
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</record>

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Genetic transformation of Citrus sinensis cv. Hamlin with hrpN gene from Erwinia amylovora and evaluation of the transgenic lines for resistance to citrus canker

Genetic transformation of Citrus sinensis cv. Hamlin with hrpN gene from Erwinia amylovora and evaluation of the transgenic lines for resistance to citrus canker

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri